Hoobkas nqi qis Alkaline ProteinaseK CAS 39450-01-6
Peb cog lus tias yuav muab cov nqi sib tw, cov khoom lag luam zoo tshaj plaws thiab cov kev daws teeb meem zoo, thiab xa khoom sai rau lub Hoobkas tus nqi qis Alkaline.ProteinaseK CAS 39450-01-6, Peb tau mob siab xav ua ntej los koom tes nrog cov neeg siv khoom thoob plaws ntiaj teb. Peb ntseeg tias peb yuav ua rau koj txaus siab. Peb kuj txais tos cov neeg yuav khoom tuaj rau peb lub koom haum thiab yuav peb cov khoom.
Peb cog lus tias yuav muab cov nqi sib tw, cov khoom lag luam zoo tshaj plaws thiab cov kev daws teeb meem zoo, thiab xa khoom sai rauTuam Tshoj Alkaline ProteinaseK thiab 39450-01-6, Vim yog qhov zoo thiab tus nqi tsim nyog, peb cov khoom lag luam tau raug xa tawm mus rau ntau dua 10 lub tebchaws thiab thaj chaw. Peb tab tom nrhiav kev koom tes nrog txhua tus neeg siv khoom los ntawm tsev thiab txawv teb chaws. Ntxiv mus, kev txaus siab ntawm cov neeg siv khoom yog peb txoj kev nrhiav mus ib txhis.
| Kua No.1 | |
| Kev ua haujlwm tshwj xeeb ntawm cov protein> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
| Hmoov No.2 | |
| Qhov tsos | Hmoov dawb lyophilized |
| Kev huv ntawm electrophoretic | ≥95% |
| Kev ua haujlwm ntawm cov enzyme | ≥30U/mg |
| Cov seem ntawm cov kua qaub nucleic | Tsis pom |
| Cov seem ntawm Deoxyribonuclease | Tsis pom |
| Cov seem ntawm ribonuclease | Tsis pom |
| Tus lej ntawm lub laub | G1205-10ML |
| Kev Khaws Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia ua haujlwm tsawg kawg 1 xyoos, kev thauj mus los hauv lub hnab dej khov. |
Proteinase Kyog ib qho serine protease uas yog ib feem ntawm tsev neeg subtilisin nrog kev ua haujlwm enzymatic zoo thiab dav substrate specificity, uas feem ntau decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas feem ntau siv los rhuav tshem cov protein kom tsim cov peptides luv luv. Proteinase K muaj tus yam ntxwv ntawm cov catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob lub Ca2 + binding sites nyob ib puag ncig lub chaw nquag kom nce nws qhov ruaj khov thiab tswj cov enzymatic ua haujlwm siab nyob rau hauv ntau yam mob.
1. Cov khoom siv kuaj mob caj ces.
2. Cov khoom siv rho tawm RNA thiab DNA.
3. Tshem tawm cov khoom tsis yog protein hauv cov ntaub so ntswg thiab rhuav tshem cov protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Kev tshem tawm sab hnub poob.
6. Kev tsim kho thiab kev tsim cov enzymatic glycated albumin reagents hauv kev kuaj mob hauv vitro.
1. 30mg/lub raj mis 1g/lub raj mis los yog 100g/lub raj mis
2. 1kgs / hnab ntawv ci
Proteinase K yog ib qho serine protease ruaj khov nrog rau qhov tshwj xeeb dav dav ntawm cov substrate. Nws rhuav tshem ntau cov protein hauv lub xeev ib txwm txawm tias nyob hauv qhov muaj cov tshuaj ntxuav. Nws tsis raug ua haujlwm los ntawm cov hlau ions, cov neeg sawv cev chelating (piv txwv li, EDTA), sulfhydryl reagents, lossis los ntawm tripsin lossis chymotrypsin inhibitors. Nws ruaj khov dhau ntawm ntau pH (4–12.5), nrog rau kev ua haujlwm zoo tshaj plaws ntawm pH 6.5–9.5. Kev ua haujlwm tuaj yeem raug txhawb los ntawm kev ntxiv cov neeg sawv cev denaturing (SDS thiab Urea). Kev denaturation sai ntawm cov enzyme tshwm sim ntawm qhov kub siab dua 70 ° C. Autolysis ntawm cov enzyme tshwm sim ntau dua ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis raug ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua haujlwm proteolytic tag nrho, txawm tias tom qab autolysis ntau heev. Proteinase K feem ntau siv rau hauv cov ntawv thov molecular biology los zom cov protein tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob me me, cov hlwb cog qoob loo, thiab cov nroj tsuag. Feem ntau cov enzyme no siv ntawm 50–200 ug/ml hauv cov tshuaj nucleic acid ntawm pH 7.5–8.0 thiab 37~55oC. Lub sijhawm incubation sib txawv ntawm 30 feeb txog 18 teev.
Ib qho serine protease ruaj khov thiab muaj zog heev; siv rau kev cais cov protein thiab nucleic acid. Proteinase K yog siv rau hauv kev ntxuav RNA thiab DNA los ntawm cov ntaub so ntswg lossis cov kab cell.
Peb cog lus tias yuav muab cov nqi sib tw, cov khoom lag luam zoo tshaj plaws thiab cov kev daws teeb meem zoo, thiab xa khoom sai rau lub Hoobkas tus nqi qis Alkaline ProteinaseK.CAS39450-01-6, Peb tau mob siab xav ua ntej los koom tes nrog cov neeg siv khoom thoob plaws ntiaj teb. Peb ntseeg tias peb yuav ua rau koj txaus siab. Peb kuj txais tos cov neeg yuav khoom tuaj rau peb lub koom haum thiab yuav peb cov khoom.
Hoobkas nqi qisTuam Tshoj Alkaline ProteinaseK thiab 39450-01-6, Vim yog qhov zoo thiab tus nqi tsim nyog, peb cov khoom lag luam tau raug xa tawm mus rau ntau dua 10 lub tebchaws thiab thaj chaw. Peb tab tom nrhiav kev koom tes nrog txhua tus neeg siv khoom los ntawm tsev thiab txawv teb chaws. Ntxiv mus, kev txaus siab ntawm cov neeg siv khoom yog peb txoj kev nrhiav mus ib txhis.
