Kev Tsim Qauv Zoo Tshaj Plaws Proteinase K CAS No. 39450-01-6 nrog Tus Nqi Zoo Tshaj Plaws
Peb feem ntau ua haujlwm ua ib pab neeg ua haujlwm kom paub tseeb tias peb yuav muab qhov zoo tshaj plaws thiab tus nqi zoo tshaj plaws rau Kev Tsim Qauv Zoo Tshaj Plaws.Proteinase K CAS No. 39450-01-6nrog Tus Nqi Zoo Tshaj Plaws, Yog xav paub ntxiv thiab qhov tseeb, thov nrog peb tham sai li sai tau!
Peb feem ntau ua haujlwm ua ib pab neeg ua haujlwm kom paub tseeb tias peb yuav muab qhov zoo tshaj plaws thiab tus nqi muag zoo tshaj plaws rau koj.Tuam Tshoj Tritirachium Album Limber thiab Molecular Biology, Kev tswj xyuas zoo nruj heev raug ua tiav hauv txhua qhov txuas ntawm tag nrho cov txheej txheem ntau lawm. Peb vam tias yuav tsim kom muaj kev koom tes zoo thiab sib pab nrog koj. Raws li cov kev daws teeb meem zoo thiab kev pabcuam ua ntej muag / tom qab muag zoo meej yog peb lub tswv yim, qee tus neeg siv khoom tau koom tes nrog peb ntau dua 5 xyoos.
| Kua No.1 | |
| Kev ua haujlwm tshwj xeeb ntawm cov protein> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
| Hmoov No.2 | |
| Qhov tsos | Hmoov dawb lyophilized |
| Kev huv ntawm electrophoretic | ≥95% |
| Kev ua haujlwm ntawm cov enzyme | ≥30U/mg |
| Cov seem ntawm cov kua qaub nucleic | Tsis pom |
| Cov seem ntawm Deoxyribonuclease | Tsis pom |
| Cov seem ntawm ribonuclease | Tsis pom |
| Tus lej ntawm lub laub | G1205-10ML |
| Kev Khaws Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia ua haujlwm tsawg kawg 1 xyoos, kev thauj mus los hauv lub hnab dej khov. |
Proteinase Kyog ib qho serine protease uas yog ib feem ntawm tsev neeg subtilisin nrog kev ua haujlwm enzymatic zoo thiab dav substrate specificity, uas feem ntau decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas feem ntau siv los rhuav tshem cov protein kom tsim cov peptides luv luv.Proteinase Kmuaj tus yam ntxwv ntawm cov catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob lub Ca2 + khi qhov chaw nyob ib puag ncig lub chaw nquag kom nce nws qhov ruaj khov thiab tswj hwm kev ua haujlwm enzymatic siab nyob rau hauv ntau yam mob.
1. Cov khoom siv kuaj mob caj ces.
2. Cov khoom siv rho tawm RNA thiab DNA.
3. Tshem tawm cov khoom tsis yog protein hauv cov ntaub so ntswg thiab rhuav tshem cov protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Kev tshem tawm sab hnub poob.
6. Kev tsim kho thiab kev tsim cov enzymatic glycated albumin reagents hauv kev kuaj mob hauv vitro.
1. 30mg/lub raj mis 1g/lub raj mis los yog 100g/lub raj mis
2. 1kgs / hnab ntawv ci
Proteinase K yog ib qho serine protease ruaj khov nrog rau qhov tshwj xeeb dav dav ntawm cov substrate. Nws rhuav tshem ntau cov protein hauv lub xeev ib txwm txawm tias nyob hauv qhov muaj cov tshuaj ntxuav. Nws tsis raug ua haujlwm los ntawm cov hlau ions, cov neeg sawv cev chelating (piv txwv li, EDTA), sulfhydryl reagents, lossis los ntawm tripsin lossis chymotrypsin inhibitors. Nws ruaj khov dhau ntawm ntau pH (4–12.5), nrog rau kev ua haujlwm zoo tshaj plaws ntawm pH 6.5–9.5. Kev ua haujlwm tuaj yeem raug txhawb los ntawm kev ntxiv cov neeg sawv cev denaturing (SDS thiab Urea). Kev denaturation sai ntawm cov enzyme tshwm sim ntawm qhov kub siab dua 70 ° C. Autolysis ntawm cov enzyme tshwm sim ntau dua ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis raug ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua haujlwm proteolytic tag nrho, txawm tias tom qab autolysis ntau heev. Proteinase K feem ntau siv rau hauv cov ntawv thov molecular biology los zom cov protein tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob me me, cov hlwb cog qoob loo, thiab cov nroj tsuag. Feem ntau cov enzyme no siv ntawm 50–200 ug/ml hauv cov tshuaj nucleic acid ntawm pH 7.5–8.0 thiab 37~55oC. Lub sijhawm incubation sib txawv ntawm 30 feeb txog 18 teev.
Ib qho serine protease ruaj khov thiab muaj zog heev; siv rau kev cais cov protein thiab nucleic acid. Proteinase K yog siv rau hauv kev ntxuav RNA thiab DNA los ntawm cov ntaub so ntswg lossis cov kab cell.
Peb feem ntau ua haujlwm ua ib pab neeg ua haujlwm kom paub tseeb tias peb yuav muab qhov zoo tshaj plaws thiab tus nqi muag zoo tshaj plaws rau Kev Tsim Qauv Zoo Proteinase K CAS No. 39450-01-6 nrog Tus Nqi Zoo Tshaj Plaws, Yog xav paub ntxiv thiab cov ntaub ntawv, thov hu rau peb sai li sai tau!
Kev Tsim Qauv Tshaj LijTuam Tshoj Tritirachium Album Limber thiab Molecular Biology, Kev tswj xyuas zoo nruj heev raug ua tiav hauv txhua qhov txuas ntawm tag nrho cov txheej txheem ntau lawm. Peb vam tias yuav tsim kom muaj kev koom tes zoo thiab sib pab nrog koj. Raws li cov kev daws teeb meem zoo thiab kev pabcuam ua ntej muag / tom qab muag zoo meej yog peb lub tswv yim, qee tus neeg siv khoom tau koom tes nrog peb ntau dua 5 xyoos.
