Professional Design High Quality Proteinase K CAS No. 39450-01-6 nrog tus nqi zoo tshaj
Peb feem ntau ua tau ib tug neeg ua hauj lwm tangible ua kom paub tseeb tias peb yuav muab koj cov txiaj ntsig zoo tshaj plaws ntxiv rau tus nqi muag zoo tshaj plaws rau Kev Tsim Kho Siab ZooProteinase K CAS Nr . 39450-01-6Nrog Tus Nqi Zoo Tshaj Plaws, Xav paub ntxiv thiab qhov tseeb, thov hais rau peb sai li sai tau!
Peb feem ntau ua tau ib tug neeg ua hauj lwm tangible ua kom paub tseeb tias peb yuav muab koj cov txiaj ntsig zoo tshaj plaws ntxiv rau tus nqi muag zoo tshaj plaws rauTuam Tshoj Tritirachium Album Limber thiab Molecular Biology, Kev tswj xyuas nruj nruj yog raug tua nyob rau hauv txhua qhov txuas ntawm tag nrho cov txheej txheem ntau lawm.Peb vam tias yuav tsim kom muaj kev sib raug zoo thiab kev sib koom tes nrog koj. Raws li cov kev daws teeb meem zoo thiab zoo meej ua ntej muag / tom qab-muag kev pabcuam yog peb lub tswv yim, qee cov neeg siv khoom tau koom tes nrog peb rau ntau tshaj 5 xyoos.
| No.1 kua | |
| Protein kev ua haujlwm> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
| No.2 hmoov | |
| Qhov tshwm sim | Dawb lyophilized hmoov |
| Electrophoretic purity | ≥95% |
| Kev ua haujlwm enzyme | ≥ 30 U / mg |
| Nucleic acid residue | Pom tsis pom |
| Deoxyribonuclease residue | Pom tsis pom |
| Ribonuclease residue | Pom tsis pom |
| Lub laub No. | G1205-10ML |
| Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia kev ua haujlwm tsawg kawg yog 1 xyoos, kev thauj mus los hauv hnab khov. |
Proteinase Kyog ib tug serine protease teej tug mus rau tsev neeg subtilisin nrog npaum enzymatic kev ua si thiab broad substrate tshwj xeeb, uas nyiam decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas yog. feem ntau siv los degrade proteins los tsim peptides luv. Proteinase K muaj tus yam ntxwv ntawm cov kab mob catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob qhov chaw Ca2+ khi nyob ib ncig ntawm lub chaw nquag ua kom nws ruaj khov thiab tswj cov kev ua kom muaj zog hauv ntau yam kev mob.
1. Cov khoom siv kuaj kab mob caj ces.
2. RNA thiab DNA rho cov khoom siv.
3. Extract cov khoom tsis muaj protein nyob hauv cov ntaub so ntswg thiab degrade protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Western blotting.
6. Kev txhim kho thiab tsim cov enzymatic glycated albumin reagents nyob rau hauv kev kuaj mob hauv vitro.
1. 30mg / lub raj mis 1g / lub raj mis lossis 100g / lub raj mis
2.1kgs / ntawv ci hnab
Proteinase K yog ib qho ruaj khov serine protease nrog dav substrate tshwj xeeb. Nws degrades ntau proteins nyob rau hauv ib txwm lub xeev txawm nyob rau hauv lub xub ntiag ntawm detergents. Nws tsis yog qhib los ntawm cov hlau ions, chelate agents (piv txwv li, EDTA), sulfhydryl reagents, los yog tripsin los yog chymotrypsin inhibitors. Nws ruaj khov tshaj pH dav (4-12.5), nrog rau kev ua haujlwm zoo ntawm pH 6.5-9.5. Kev ua ub no tuaj yeem txhawb nqa los ntawm kev ntxiv cov tshuaj denaturing (SDS thiab Urea). Kev nrawm nrawm ntawm cov enzyme tshwm sim ntawm qhov kub siab tshaj 70 ° C. Autolysis ntawm enzyme tshwm sim nce ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua tiav proteolytic, txawm tias tom qab nws siv ntau autolysis. Proteinase K feem ntau siv rau hauv kev siv tshuaj lom neeg molecular los zom cov proteins uas tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob, kab lis kev cai, thiab cov nroj tsuag. Cov enzyme feem ntau yog siv ntawm 50-200 ug / ml hauv nucleic acid npaj ntawm pH 7.5-8.0 thiab 37 ~ 55oC. Lub sij hawm incubation txawv ntawm 30 feeb mus rau 18 teev.
Ib qho ruaj khov thiab muaj zog heev serine protease; siv rau kev sib cais ntawm cov protein thiab nucleic acid.Proteinase K yog siv los ua kom huv ntawm RNA thiab DNA los ntawm cov ntaub so ntswg lossis kab ntawm tes.
Peb feem ntau ua ib tug neeg ua hauj lwm tangible ua kom paub tseeb tias peb yuav muab koj cov txiaj ntsig zoo tshaj plaws ntxiv rau tus nqi muag zoo tshaj plaws rau Kev Tsim Kho Zoo Tshaj Plaws Proteinase K CAS No. 39450-01-6 nrog Tus Nqi Zoo, Xav paub ntxiv thiab qhov tseeb, thov hais rau peb kom sai li sai tau!
Professional DesignTuam Tshoj Tritirachium Album Limber thiab Molecular Biology, Kev tswj xyuas nruj nruj yog raug tua nyob rau hauv txhua qhov txuas ntawm tag nrho cov txheej txheem ntau lawm.Peb vam tias yuav tsim kom muaj kev sib raug zoo thiab kev sib koom tes nrog koj. Raws li cov kev daws teeb meem zoo thiab zoo meej ua ntej muag / tom qab-muag kev pabcuam yog peb lub tswv yim, qee cov neeg siv khoom tau koom tes nrog peb rau ntau tshaj 5 xyoos.
