ODM Hoobkas Proteinase K CAS No. 39450-01-6 Kev Txhim Kho Pob Txha
Peb lub hom phiaj yog los pab peb cov neeg siv khoom thiab cov neeg siv khoom zoo tshaj plaws thiab cov khoom sib tw portable digital rau ODM Hoobkas Proteinase K CAS No. 39450-01-6 rau Txhim Kho Pob Txha Lub Zog, Peb xav tias koj yuav zoo siab nrog peb cov nqi muag khoom, sab saum toj. kev daws teeb meem zoo thiab xa khoom sai. Peb cia siab tias koj tuaj yeem muab sijhawm rau peb los muab rau koj thiab ua koj tus khub zoo tshaj plaws!
Peb lub hom phiaj yog los pab peb cov neeg siv khoom thiab cov neeg siv khoom zoo tshaj plaws thiab kev sib tw portable digital khoom rauTuam Tshoj Chemicals Khoom thiab Raw Materials, Lawv yog cov qauv ruaj khov thiab txhawb nqa zoo thoob plaws lub ntiaj teb. Tsis txhob ploj mus ua haujlwm loj hauv lub sijhawm ceev, nws yog ib qho yuav tsum tau rau koj tus kheej ntawm qhov zoo heev. Coj los ntawm lub hauv paus ntsiab lus ntawm Prudence, Efficiency, Union thiab Innovation. lub koom haum. ua ib qho kev siv zog zoo los nthuav nws txoj kev lag luam thoob ntiaj teb, tsa nws lub koom haum. rofit thiab nce nws export scale. Peb tau ntseeg siab tias peb yuav muaj lub zeem muag ci ntsa iab thiab nthuav tawm thoob plaws ntiaj teb nyob rau xyoo tom ntej.
No.1 kua | |
Protein kev ua haujlwm> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
No.2 hmoov | |
Qhov tshwm sim | Dawb lyophilized hmoov |
Electrophoretic purity | ≥95% |
Kev ua haujlwm enzyme | ≥ 30 U / mg |
Nucleic acid residue | Pom tsis pom |
Deoxyribonuclease residue | Pom tsis pom |
Ribonuclease residue | Pom tsis pom |
Lub laub No. | G1205-10ML |
Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia kev ua haujlwm tsawg kawg yog 1 xyoos, kev thauj mus los hauv hnab khov. |
Proteinase K yog ib tug serine protease belongs rau tsev neeg subtilisin nrog npaum enzymatic kev ua si thiab broad substrate specifications, uas nyiam decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas feem ntau siv los degrade proteins los tsim peptides luv. Proteinase K muaj tus yam ntxwv ntawm cov kab mob catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob qhov chaw Ca2+ khi nyob ib ncig ntawm lub chaw nquag ua kom nws ruaj khov thiab tswj cov kev ua kom muaj zog hauv ntau yam kev mob.
1. Cov khoom siv kuaj kab mob caj ces.
2. RNA thiab DNA rho cov khoom siv.
3. Extract cov khoom tsis muaj protein nyob hauv cov ntaub so ntswg thiab degrade protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Western blotting.
6. Kev txhim kho thiab tsim cov enzymatic glycated albumin reagents nyob rau hauv kev kuaj mob hauv vitro.
1. 30mg / lub raj mis 1g / lub raj mis lossis 100g / lub raj mis
2.1kgs / ntawv ci hnab
Proteinase K yog ib qho ruaj khov serine protease nrog dav substrate tshwj xeeb. Nws degrades ntau proteins nyob rau hauv ib txwm lub xeev txawm nyob rau hauv lub xub ntiag ntawm detergents. Nws tsis yog qhib los ntawm cov hlau ions, chelate agents (piv txwv li, EDTA), sulfhydryl reagents, los yog tripsin los yog chymotrypsin inhibitors. Nws ruaj khov tshaj pH dav (4-12.5), nrog rau kev ua haujlwm zoo ntawm pH 6.5-9.5. Kev ua ub no tuaj yeem txhawb nqa los ntawm kev ntxiv cov tshuaj denaturing (SDS thiab Urea). Kev nrawm nrawm ntawm cov enzyme tshwm sim ntawm qhov kub siab tshaj 70 ° C. Autolysis ntawm enzyme tshwm sim nce ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua tiav proteolytic, txawm tias tom qab nws siv ntau autolysis. Proteinase K feem ntau siv rau hauv kev siv tshuaj lom neeg molecular los zom cov proteins uas tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob, kab lis kev cai, thiab cov nroj tsuag. Cov enzyme feem ntau yog siv ntawm 50-200 ug / ml hauv nucleic acid npaj ntawm pH 7.5-8.0 thiab 37 ~ 55oC. Lub sij hawm incubation txawv ntawm 30 feeb mus rau 18 teev.
Ib qho ruaj khov thiab muaj zog heev serine protease; siv rau kev sib cais ntawm cov protein thiab nucleic acid.Proteinase K yog siv los ua kom huv ntawm RNA thiab DNA los ntawm cov ntaub so ntswg lossis kab ntawm tes.
Peb lub hom phiaj yog los pab peb cov neeg siv khoom thiab cov neeg siv khoom zoo tshaj plaws thiab cov khoom sib tw portable digital rau ODM Hoobkas Proteinase K CAS No. 39450-01-6 rau Txhim Kho Pob Txha Lub Zog, Peb xav tias koj yuav zoo siab nrog peb cov nqi muag khoom, sab saum toj. kev daws teeb meem zoo thiab xa khoom sai. Peb cia siab tias koj tuaj yeem muab sijhawm rau peb los muab rau koj thiab ua koj tus khub zoo tshaj plaws!
ODM HoobkasTuam Tshoj Chemicals Khoom thiab Raw Materials, Lawv yog cov qauv ruaj khov thiab txhawb nqa zoo thoob plaws lub ntiaj teb. Tsis txhob ploj mus ua haujlwm loj hauv lub sijhawm ceev, nws yog ib qho yuav tsum tau rau koj tus kheej ntawm qhov zoo heev. Coj los ntawm lub hauv paus ntsiab lus ntawm Prudence, Efficiency, Union thiab Innovation. lub koom haum. ua ib qho kev siv zog zoo los nthuav nws txoj kev lag luam thoob ntiaj teb, tsa nws lub koom haum. rofit thiab nce nws export scale. Peb tau ntseeg siab tias peb yuav muaj lub zeem muag ci ntsa iab thiab nthuav tawm thoob plaws ntiaj teb nyob rau xyoo tom ntej.