Lub koob npe nrov Kub Muag Proteinase K CAS 39450-01-6 Zoo Guaranteed
Peb xav tias cov neeg siv khoom xav li cas, qhov nrawm nrawm los ua haujlwm hauv kev txaus siab ntawm tus neeg siv khoom txoj haujlwm ntawm lub hauv paus ntsiab lus, tso cai rau kev ua haujlwm zoo dua qub, txo cov nqi ua haujlwm, cov nqi tsim nyog ntxiv, yeej cov neeg siv khoom tshiab thiab yav dhau los kev txhawb nqa thiab kev lees paub rau Lub koob npe nrov Kub MuagProteinase K CAS 39450-01-6Zoo Guaranteed, Raws li tus kws tshaj lij tshwj xeeb hauv daim teb no, peb tau cog lus los daws txhua qhov teeb meem ntawm kev tiv thaiv kub kub rau cov neeg siv.
Peb xav tias cov neeg siv khoom xav li cas, qhov nrawm nrawm los ua haujlwm hauv kev txaus siab ntawm tus neeg siv khoom txoj haujlwm ntawm lub hauv paus ntsiab lus, tso cai rau kev ua haujlwm zoo dua qub, txo cov nqi ua haujlwm, cov nqi tsim nyog ntxiv, yeej cov neeg siv khoom tshiab thiab yav dhau los kev txhawb nqa thiab kev lees paub rauTuam Tshoj Proteinase K thiab CAS 39450-01-6, Muaj cov khoom tsim & ua tiav thiab cov neeg ua haujlwm txawj ua haujlwm kom ntseeg tau cov khoom zoo. Peb tau pom qhov zoo tshaj plaws ua ntej-muag, muag, kev pab cuam tom qab muag kom ntseeg tau tias cov neeg siv khoom tuaj yeem tau txais kev lees paub los txiav txim. Txog rau tam sim no peb cov kev daws teeb meem tam sim no tsiv mus sai thiab nrov heev hauv South America, East Asia, Middle East, Africa, thiab lwm yam.
| No.1 kua | |
| Protein kev ua haujlwm> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
| No.2 hmoov | |
| Qhov tshwm sim | Dawb lyophilized hmoov |
| Electrophoretic purity | ≥95% |
| Kev ua haujlwm enzyme | ≥ 30 U / mg |
| Nucleic acid residue | Pom tsis pom |
| Deoxyribonuclease residue | Pom tsis pom |
| Ribonuclease residue | Pom tsis pom |
| Lub laub No. | G1205-10ML |
| Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia kev ua haujlwm tsawg kawg yog 1 xyoos, kev thauj mus los hauv hnab khov. |
Proteinase Kyog ib tug serine protease teej tug mus rau tsev neeg subtilisin nrog npaum enzymatic kev ua si thiab broad substrate tshwj xeeb, uas nyiam decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas yog. feem ntau siv los degrade proteins los tsim peptides luv.Proteinase Kmuaj cov yam ntxwv ntawm cov kab mob catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob lub Ca2+ khi qhov chaw nyob ib ncig ntawm lub chaw nquag ua kom nws ruaj khov thiab tswj cov kev ua kom muaj zog hauv ntau yam kev mob.
1. Cov khoom siv kuaj kab mob caj ces.
2. RNA thiab DNA rho cov khoom siv.
3. Extract cov khoom tsis muaj protein nyob hauv cov ntaub so ntswg thiab degrade protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Western blotting.
6. Kev txhim kho thiab tsim cov enzymatic glycated albumin reagents nyob rau hauv kev kuaj mob hauv vitro.
1. 30mg / lub raj mis 1g / lub raj mis lossis 100g / lub raj mis
2.1kgs / ntawv ci hnab
Proteinase K yog ib qho ruaj khov serine protease nrog dav substrate tshwj xeeb. Nws degrades ntau proteins nyob rau hauv ib txwm lub xeev txawm nyob rau hauv lub xub ntiag ntawm detergents. Nws tsis yog qhib los ntawm cov hlau ions, chelate agents (piv txwv li, EDTA), sulfhydryl reagents, los yog tripsin los yog chymotrypsin inhibitors. Nws ruaj khov tshaj pH dav (4-12.5), nrog rau kev ua haujlwm zoo ntawm pH 6.5-9.5. Kev ua ub no tuaj yeem txhawb nqa los ntawm kev ntxiv cov tshuaj denaturing (SDS thiab Urea). Kev nrawm nrawm ntawm cov enzyme tshwm sim ntawm qhov kub siab tshaj 70 ° C. Autolysis ntawm enzyme tshwm sim nce ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua tiav proteolytic, txawm tias tom qab nws siv ntau autolysis. Proteinase K feem ntau siv rau hauv kev siv tshuaj lom neeg molecular los zom cov proteins uas tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob, kab lis kev cai, thiab cov nroj tsuag. Cov enzyme feem ntau yog siv ntawm 50-200 ug / ml hauv nucleic acid npaj ntawm pH 7.5-8.0 thiab 37 ~ 55oC. Lub sij hawm incubation txawv ntawm 30 feeb mus rau 18 teev.
Ib qho ruaj khov thiab muaj zog heev serine protease; siv rau kev sib cais ntawm cov protein thiab nucleic acid.Proteinase K yog siv los ua kom huv ntawm RNA thiab DNA los ntawm cov ntaub so ntswg lossis kab ntawm tes.
Peb xav tias cov neeg siv khoom xav li cas, qhov nrawm nrawm los ua haujlwm hauv kev txaus siab ntawm tus neeg siv khoom txoj haujlwm ntawm lub hauv paus ntsiab lus, tso cai rau kev ua haujlwm zoo dua qub, txo cov nqi ua haujlwm, cov nqi tsim nyog ntxiv, yeej cov neeg siv khoom tshiab thiab yav dhau los kev txhawb nqa thiab kev lees paub rau Lub koob npe nrov Kub Muag Proteinase K CAS39450-01-6Zoo Guaranteed, Raws li tus kws tshaj lij tshwj xeeb hauv daim teb no, peb tau cog lus los daws txhua qhov teeb meem ntawm kev tiv thaiv kub kub rau cov neeg siv.
Lub koob npe nrovTuam Tshoj Proteinase K thiab CAS 39450-01-6, Muaj cov khoom tsim & ua tiav thiab cov neeg ua haujlwm txawj ua haujlwm kom ntseeg tau cov khoom zoo. Peb tau pom qhov zoo tshaj plaws ua ntej-muag, muag, kev pab cuam tom qab muag kom ntseeg tau tias cov neeg siv khoom tuaj yeem tau txais kev lees paub los txiav txim. Txog rau tam sim no peb cov kev daws teeb meem tam sim no tsiv mus sai thiab nrov heev hauv South America, East Asia, Middle East, Africa, thiab lwm yam.
