Factory Supply High Purity White Powder Proteinase K CAS 39450-01-6 Kub Muag ntawm Tus Nqi qis tshaj
Ua raws li koj txoj kev xav ntawm "Tsim cov kev daws teeb meem zoo tshaj plaws thiab ua phooj ywg nrog cov neeg los ntawm thoob plaws lub ntiaj teb", peb ib txwm teeb tsa lub siab nyiam ntawm cov neeg siv khoom los pib nrog rau Lub Hoobkas Khoom Siv High Purity Dawb HmoovProteinase K CAS Nr .: 39450-01-6nyob rau hauv Kub Muag ntawm tus nqi qis tshaj, Peb muaj kev koom tes sib sib zog nqus nrog ntau pua lub Hoobkas nyob ze rau Tuam Tshoj. Cov khoom uas peb nthuav qhia tuaj yeem sib phim nrog cov npe sib txawv. Xaiv peb, thiab peb yuav tsis ua rau koj khuv xim!
Ua raws li koj txoj kev xav ntawm "Tsim cov kev daws teeb meem zoo tshaj plaws thiab ua phooj ywg nrog cov neeg los ntawm thoob plaws lub ntiaj teb", peb ib txwm teeb tsa lub siab nyiam ntawm cov neeg siv khoom pib nrog rauTuam Tshoj Proteinase K / Fluvoxamine thiab Proteinase K Powder, Peb tenet yog "kev ncaj ncees ua ntej, zoo tshaj plaws". Tam sim no peb muaj kev ntseeg siab los muab kev pabcuam zoo thiab cov khoom zoo rau koj. Peb cia siab tias peb tuaj yeem tsim kom muaj kev sib koom tes ua lag luam nrog koj yav tom ntej!
| No.1 kua | |
| Protein kev ua haujlwm> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
| No.2 hmoov | |
| Qhov tshwm sim | Dawb lyophilized hmoov |
| Electrophoretic purity | ≥95% |
| Kev ua haujlwm enzyme | ≥ 30 U / mg |
| Nucleic acid residue | Pom tsis pom |
| Deoxyribonuclease residue | Pom tsis pom |
| Ribonuclease residue | Pom tsis pom |
| Lub laub No. | G1205-10ML |
| Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia kev ua haujlwm tsawg kawg yog 1 xyoos, kev thauj mus los hauv hnab khov. |
Proteinase Kyog ib tug serine protease teej tug mus rau tsev neeg subtilisin nrog npaum enzymatic kev ua si thiab broad substrate tshwj xeeb, uas nyiam decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas yog. feem ntau siv los degrade proteins los tsim peptides luv. Proteinase K muaj tus yam ntxwv ntawm cov kab mob catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob qhov chaw Ca2+ khi nyob ib ncig ntawm lub chaw nquag ua kom nws ruaj khov thiab tswj cov kev ua kom muaj zog hauv ntau yam kev mob.
1. Cov khoom siv kuaj kab mob caj ces.
2. RNA thiab DNA rho cov khoom siv.
3. Extract cov khoom tsis muaj protein nyob hauv cov ntaub so ntswg thiab degrade protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Western blotting.
6. Kev txhim kho thiab tsim cov enzymatic glycated albumin reagents nyob rau hauv kev kuaj mob hauv vitro.
1. 30mg / lub raj mis 1g / lub raj mis lossis 100g / lub raj mis
2.1kgs / ntawv ci hnab
Proteinase K yog ib qho ruaj khov serine protease nrog dav substrate tshwj xeeb. Nws degrades ntau proteins nyob rau hauv ib txwm lub xeev txawm nyob rau hauv lub xub ntiag ntawm detergents. Nws tsis yog qhib los ntawm cov hlau ions, chelate agents (piv txwv li, EDTA), sulfhydryl reagents, los yog tripsin los yog chymotrypsin inhibitors. Nws ruaj khov tshaj pH dav (4-12.5), nrog rau kev ua haujlwm zoo ntawm pH 6.5-9.5. Kev ua ub no tuaj yeem txhawb nqa los ntawm kev ntxiv cov tshuaj denaturing (SDS thiab Urea). Kev nrawm nrawm ntawm cov enzyme tshwm sim ntawm qhov kub siab tshaj 70 ° C. Autolysis ntawm enzyme tshwm sim nce ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua tiav proteolytic, txawm tias tom qab nws siv ntau autolysis. Proteinase K feem ntau siv rau hauv kev siv tshuaj lom neeg molecular los zom cov proteins uas tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob, kab lis kev cai, thiab cov nroj tsuag. Cov enzyme feem ntau yog siv ntawm 50-200 ug / ml hauv nucleic acid npaj ntawm pH 7.5-8.0 thiab 37 ~ 55oC. Lub sij hawm incubation txawv ntawm 30 feeb mus rau 18 teev.
Ib qho ruaj khov thiab muaj zog heev serine protease; siv rau kev sib cais ntawm cov protein thiab nucleic acid.Proteinase K yog siv los ua kom huv ntawm RNA thiab DNA los ntawm cov ntaub so ntswg lossis kab ntawm tes.
Ua raws li koj txoj kev xav ntawm "Tsim cov kev daws teeb meem zoo tshaj plaws thiab ua phooj ywg nrog cov neeg los ntawm thoob plaws lub ntiaj teb", peb ib txwm teeb tsa qhov kev xav tau ntawm cov neeg siv khoom pib nrog rau Hoobkas Khoom Siv High Purity Dawb Powder Proteinase K CAS 39450-01-6 hauv Kub Muag ntawm tus nqi qis tshaj, Peb muaj kev koom tes sib sib zog nqus nrog ntau pua lub Hoobkas nyob ze rau Tuam Tshoj. Cov khoom uas peb nthuav qhia tuaj yeem sib phim nrog cov npe sib txawv. Xaiv peb, thiab peb yuav tsis ua rau koj khuv xim!
Factory SupplyTuam Tshoj Proteinase K / Fluvoxamine thiab Proteinase K Powder, Peb tenet yog "kev ncaj ncees ua ntej, zoo tshaj plaws". Tam sim no peb muaj kev ntseeg siab los muab kev pabcuam zoo thiab cov khoom zoo rau koj. Peb cia siab tias peb tuaj yeem tsim kom muaj kev sib koom tes ua lag luam nrog koj yav tom ntej!
