Hoobkas nqi qis Alkaline ProteinaseK CAS 39450-01-6
Peb tau cog lus los muab cov nqi sib tw, cov khoom tshwj xeeb thiab cov kev daws teeb meem zoo, ib yam nkaus li xa khoom sai rau lub Hoobkas nqi qis AlkalineProteinaseK CAS 39450-01-6, Peb tau ua siab dawb xav ua ntej los koom tes nrog cov neeg siv khoom txhua qhov chaw hauv ntiaj teb. Peb ntseeg tias peb yuav txaus siab rau koj. Peb kuj zoo siab tos txais cov neeg yuav khoom mus rau peb lub koom haum thiab yuav peb cov khoom.
Peb tau cog lus los muab cov nqi sib tw, cov khoom tshwj xeeb thiab cov kev daws teeb meem zoo, ib yam nkaus li xa khoom sai rauTuam Tshoj Alkaline ProteinaseK thiab 39450-01-6, Vim yog qhov zoo thiab tus nqi tsim nyog, peb cov khoom muag tau raug xa tawm mus rau ntau tshaj 10 lub teb chaws thiab cheeb tsam. Peb tab tom tos ntsoov yuav koom tes nrog txhua tus neeg siv khoom hauv tsev thiab txawv teb chaws. Ntxiv mus, cov neeg siv khoom txaus siab yog peb txoj kev nrhiav mus ib txhis.
| No.1 kua | |
| Protein kev ua haujlwm> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
| No.2 hmoov | |
| Qhov tshwm sim | Dawb lyophilized hmoov |
| Electrophoretic purity | ≥95% |
| Kev ua haujlwm enzyme | ≥ 30 U / mg |
| Nucleic acid residue | Pom tsis pom |
| Deoxyribonuclease residue | Pom tsis pom |
| Ribonuclease residue | Pom tsis pom |
| Lub laub No. | G1205-10ML |
| Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia kev ua haujlwm tsawg kawg yog 1 xyoos, kev thauj mus los hauv hnab khov. |
Proteinase Kyog ib tug serine protease teej tug mus rau tsev neeg subtilisin nrog npaum enzymatic kev ua si thiab broad substrate tshwj xeeb, uas nyiam decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas yog. feem ntau siv los degrade proteins los tsim peptides luv. Proteinase K muaj tus yam ntxwv ntawm cov kab mob catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob qhov chaw Ca2+ khi nyob ib ncig ntawm lub chaw nquag ua kom nws ruaj khov thiab tswj cov kev ua kom muaj zog hauv ntau yam kev mob.
1. Cov khoom siv kuaj kab mob caj ces.
2. RNA thiab DNA rho cov khoom siv.
3. Extract cov khoom tsis muaj protein nyob hauv cov ntaub so ntswg thiab degrade protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Western blotting.
6. Kev txhim kho thiab tsim cov enzymatic glycated albumin reagents nyob rau hauv kev kuaj mob hauv vitro.
1. 30mg / lub raj mis 1g / lub raj mis lossis 100g / lub raj mis
2.1kgs / ntawv ci hnab
Proteinase K yog ib qho ruaj khov serine protease nrog dav substrate tshwj xeeb. Nws degrades ntau proteins nyob rau hauv ib txwm lub xeev txawm nyob rau hauv lub xub ntiag ntawm detergents. Nws tsis yog qhib los ntawm cov hlau ions, chelate agents (piv txwv li, EDTA), sulfhydryl reagents, los yog tripsin los yog chymotrypsin inhibitors. Nws ruaj khov tshaj pH dav (4-12.5), nrog rau kev ua haujlwm zoo ntawm pH 6.5-9.5. Kev ua ub no tuaj yeem txhawb nqa los ntawm kev ntxiv cov tshuaj denaturing (SDS thiab Urea). Kev nrawm nrawm ntawm cov enzyme tshwm sim ntawm qhov kub siab tshaj 70 ° C. Autolysis ntawm enzyme tshwm sim nce ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua tiav proteolytic, txawm tias tom qab nws siv ntau autolysis. Proteinase K feem ntau siv rau hauv kev siv tshuaj lom neeg molecular los zom cov proteins uas tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob, kab lis kev cai, thiab cov nroj tsuag. Cov enzyme feem ntau yog siv ntawm 50-200 ug / ml hauv nucleic acid npaj ntawm pH 7.5-8.0 thiab 37 ~ 55oC. Lub sij hawm incubation txawv ntawm 30 feeb mus rau 18 teev.
Ib qho ruaj khov thiab muaj zog heev serine protease; siv rau kev sib cais ntawm cov protein thiab nucleic acid.Proteinase K yog siv los ua kom huv ntawm RNA thiab DNA los ntawm cov ntaub so ntswg lossis kab ntawm tes.
Peb tau cog lus los muab cov nqi sib tw, cov khoom tshwj xeeb thiab cov kev daws teeb meem zoo, ib yam nkaus li xa khoom sai rau lub Hoobkas qis nqi Alkaline ProteinaseKCAS 39450-01-6, Peb tau ua siab dawb xav ua ntej los koom tes nrog cov neeg siv khoom txhua qhov chaw hauv ntiaj teb. Peb ntseeg tias peb yuav txaus siab rau koj. Peb kuj zoo siab tos txais cov neeg yuav khoom mus rau peb lub koom haum thiab yuav peb cov khoom.
Hoobkas nqi qisTuam Tshoj Alkaline ProteinaseK thiab 39450-01-6, Vim yog qhov zoo thiab tus nqi tsim nyog, peb cov khoom muag tau raug xa tawm mus rau ntau tshaj 10 lub teb chaws thiab cheeb tsam. Peb tab tom tos ntsoov yuav koom tes nrog txhua tus neeg siv khoom hauv tsev thiab txawv teb chaws. Ntxiv mus, cov neeg siv khoom txaus siab yog peb txoj kev nrhiav mus ib txhis.
