Lub Hoobkas muag zoo tshaj plaws thoob ntiaj teb Nrov Proteinase K CAS 39450-01-6 nrog Tus Nqi Tshaj Lij Tshaj Plaws thiab Hnub Kawg ntawm Kev Tsim Khoom
Txhua tus tswv cuab los ntawm peb cov neeg ua haujlwm tau txais txiaj ntsig loj ua rau muaj txiaj ntsig rau cov neeg siv khoom thiab cov koom haum kev sib txuas lus rau Hoobkas muag khoom zoo tshaj plaws thoob ntiaj tebProteinase K CAS 39450-01-6Nrog tus nqi sib kis tau zoo thiab qhov tseeb ntawm kev tsim khoom, tam sim no, peb tab tom nrhiav rau pem hauv ntej nrog kev koom tes txawv teb chaws raws cov kev sib koom tes. Nco ntsoov tias koj tuaj yeem xav tias tsis muaj nqi hu rau peb kom paub ntau ntxiv.
Txhua tus tswv cuab los ntawm peb cov neeg ua haujlwm tau txais txiaj ntsig loj ua rau muaj txiaj ntsig rau cov neeg siv khoom xav tau thiab koom haum sib txuas lus rauSuav teb 39450-01-6, Peb sov siab txais tos koj patronage thiab yuav pab tau peb cov neeg muas zaub nyob rau hauv lub tsev thiab txawv teb chaws nrog cov khoom ntawm superior zoo thiab zoo heev kev pab cuam npaj rau tus qauv ntawm kev loj hlob ntxiv raws li ib txwm. Peb ntseeg tias koj yuav tau txais txiaj ntsig los ntawm peb cov kws tshaj lij sai sai.
| No.1 kua | |
| Protein kev ua haujlwm> 800U / mL, qhov concentration yog 20mg / mL, tsis muaj Nickase residue | |
| No.2 hmoov | |
| Qhov tshwm sim | Dawb lyophilized hmoov |
| Electrophoretic purity | ≥95% |
| Kev ua haujlwm enzyme | ≥ 30 U / mg |
| Nucleic acid residue | Pom tsis pom |
| Deoxyribonuclease residue | Pom tsis pom |
| Ribonuclease residue | Pom tsis pom |
| Lub laub No. | G1205-10ML |
| Cia | Thaum khaws cia ntawm -20 ° C, cov khoom khaws cia kev ua haujlwm tsawg kawg yog 1 xyoos, kev thauj mus los hauv hnab khov. |
Proteinase Kyog ib tug serine protease teej tug mus rau tsev neeg subtilisin nrog npaum enzymatic kev ua si thiab broad substrate tshwj xeeb, uas nyiam decomposes ester bonds thiab peptides nyob ib sab ntawm C-terminus ntawm hydrophobic amino acids, sulfur-muaj amino acids, thiab aromatic amino acids bonds, uas yog. feem ntau siv los degrade proteins los tsim peptides luv. Proteinase K muaj tus yam ntxwv ntawm cov kab mob catalytic triad Asp39-His69-Ser224 tshwj xeeb rau serine proteases thiab muaj ob qhov chaw Ca2+ khi nyob ib ncig ntawm lub chaw nquag ua kom nws ruaj khov thiab tswj cov kev ua kom muaj zog hauv ntau yam kev mob.
1. Cov khoom siv kuaj kab mob caj ces.
2. RNA thiab DNA rho cov khoom siv.
3. Extract cov khoom tsis muaj protein nyob hauv cov ntaub so ntswg thiab degrade protein impurities (xws li kev npaj cov tshuaj tiv thaiv DNA thiab heparin).
4. Kev npaj ntawm Chromosomal DNA rau Pulse Electrophoresis.
5. Western blotting.
6. Kev txhim kho thiab tsim cov enzymatic glycated albumin reagents nyob rau hauv kev kuaj mob hauv vitro.
1. 30mg / lub raj mis 1g / lub raj mis lossis 100g / lub raj mis
2.1kgs / ntawv ci hnab
Proteinase K yog ib qho ruaj khov serine protease nrog dav substrate tshwj xeeb. Nws degrades ntau proteins nyob rau hauv ib txwm lub xeev txawm nyob rau hauv lub xub ntiag ntawm detergents. Nws tsis yog qhib los ntawm cov hlau ions, chelate agents (piv txwv li, EDTA), sulfhydryl reagents, los yog tripsin los yog chymotrypsin inhibitors. Nws ruaj khov tshaj pH dav (4-12.5), nrog rau kev ua haujlwm zoo ntawm pH 6.5-9.5. Kev ua ub no tuaj yeem txhawb nqa los ntawm kev ntxiv cov tshuaj denaturing (SDS thiab Urea). Kev nrawm nrawm ntawm cov enzyme tshwm sim ntawm qhov kub siab tshaj 70 ° C. Autolysis ntawm enzyme tshwm sim nce ntawm alkaline pH. Txawm li cas los xij, Proteinase K tsis ua haujlwm tag nrho los ntawm autolysis. Qee cov enzyme fragments txuas ntxiv tswj lawv cov kev ua tiav proteolytic, txawm tias tom qab nws siv ntau autolysis. Proteinase K feem ntau siv rau hauv kev siv tshuaj lom neeg molecular los zom cov proteins uas tsis xav tau, xws li nucleases hauv DNA lossis RNA npaj los ntawm cov kab mob, kab lis kev cai, thiab cov nroj tsuag. Cov enzyme feem ntau yog siv ntawm 50-200 ug / ml hauv nucleic acid npaj ntawm pH 7.5-8.0 thiab 37 ~ 55oC. Lub sij hawm incubation txawv ntawm 30 feeb mus rau 18 teev.
Ib qho ruaj khov thiab muaj zog heev serine protease; siv rau kev sib cais ntawm cov protein thiab nucleic acid.Proteinase K yog siv los ua kom huv ntawm RNA thiab DNA los ntawm cov ntaub so ntswg lossis kab ntawm tes.
Txhua tus tswv cuab los ntawm peb cov neeg ua haujlwm tau txais txiaj ntsig loj ua rau muaj txiaj ntsig rau cov neeg siv khoom xav tau thiab koom haum kev sib txuas lus rau Hoobkas zoo tshaj plaws muag thoob ntiaj teb Nrov Proteinase K CAS39450-01-6Nrog tus nqi sib kis tau zoo thiab qhov tseeb ntawm kev tsim khoom, tam sim no, peb tab tom nrhiav rau pem hauv ntej nrog kev koom tes txawv teb chaws raws cov kev sib koom tes. Nco ntsoov tias koj tuaj yeem xav tias tsis muaj nqi hu rau peb kom paub ntau ntxiv.
Hoobkas muag zoo tshaj plawsSuav teb 39450-01-6, Peb zoo siab tos txais koj cov neeg siv khoom thiab yuav pab peb cov neeg siv khoom hauv tsev thiab txawv teb chaws nrog cov khoom zoo tshaj plaws thiab cov kev pabcuam zoo tshaj plaws rau cov qauv ntawm kev txhim kho ntxiv raws li ib txwm muaj. Peb ntseeg tias koj yuav tau txais txiaj ntsig los ntawm peb cov kws tshaj lij sai sai.
